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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was uncovered to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a vital position in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were being noted but in-depth nucleotide sequence data isn't but available.
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Blocking peptides are peptides that bind especially for the target antibody and block antibody binding. These peptide generally consists of the epitope recognized from the antibody.
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This guideline outlines further attainable optimization of mobile and immunoassay parameters to ensure the absolute best outcomes are...
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This tutorial outlines additional attainable optimization of mobile and immunoassay parameters to make sure the absolute best success are...
Antibodies sure to the blocking peptide no longer bind for the epitope within the goal protein. By comparing the staining from your blocked antibody compared to the antibody by yourself, you can see which staining is particular.
Goal Info Cyclin E belongs towards the hugely conserved cyclin family, whose members are characterised by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins function as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation designs which add for the temporal coordination of each and every mitotic event. Cyclin E varieties a complex with and functions as a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is particularly degraded as cells progress check here through S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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